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Composite

Part:BBa_K539281:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-17)


rbs+crtZ(beta-carotene hydroxylase) under heat sensitive cI regulater


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1492
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This operon is under a heat sensitive cI QPI with a high promoter preceding the repressor (BBa_J23114).



Source

crtZ coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087)

References